RBTI Body Chemistry Testing

The Brix Refractometer measures the sugar content of your urine. Hold it up to any light source — a window or lamp works fine.

• Open the lid completely so it lays flat on the opposite side.
• Using a clean pipette, place a thin layer of urine on the glass window.
• Close the lid gently so it spreads the sample evenly across the glass.
• Hold the viewing end toward a light source and look through the eyepiece.
• Read the number where the blue and white sections meet. Each small line equals 0.2.
• Open the lid and rinse the glass with distilled water only. Pat dry gently.

Always start with the 5.5–8.0 strip. Read the color immediately — it changes quickly once exposed to air.

• Tear off about 2 inches of the 5.5–8.0 pH paper.
• Dip it directly into your urine specimen cup and remove it immediately.
• Compare the color to the chart on the container right away. Do not wait.
• If the color matches exactly at 5.5, also dip a strip from the 3.0–5.5 roll to check for a lower reading.

Read the color immediately after removing the strip — do not wait.

• Tear off a 1 to 1½ inch piece of the 5.5–8.0 pH paper.
• Build up saliva in the front of your mouth, insert the strip, close your lips, and immediately remove it — or spit into a small cup and dip the strip into that.
• Immediately compare the color to the chart. Do this right away.
• If the color matches at exactly 5.5, also dip a strip from the 3.0–5.5 roll.

This test dilutes your urine with distilled water so the conductivity tester can accurately measure the salt content.

• Get a clean cup and place it against a wall or something sturdy.
• Using your pipette, draw urine up to the 2 ml mark and empty it into the cup.
• Measure exactly 70 ml of distilled water and pour it into the cup with the urine.
• Turn on the EC20, place the probe in the cup, give it a gentle stir, then hold it still. Do not hold it in your hand — let it rest on its own.
• Wait approximately 1 minute for the reading to stabilize.
• Rinse the probe with distilled water only when finished.

Cell debris tells us how many dead and dying cells your body is removing through the urine. This is a visual test using your eyes and your phone flashlight.

• First, look without any light. If you can already see cloudiness or floating particles, your reading is at least 4M+.
• Shine your phone flashlight through the side of the cup and look through from the other side.
• Match what you see to the guide below, then tap Enter my reading to select it.

Prepare your urea sample and mail it to us to be tested.

• Get a clean test tube with a cap.
• Using a clean pipette held completely vertical, measure out exactly ½ ml (0.5 ml) of urine and place it in the test tube.
• Add exactly 3 ml of Universal Extraction Solution to the same test tube.
• Cap the test tube securely.
• Turn the test tube back and forth gently for a full 1 minute to mix thoroughly. A slow rocking motion works best — do not shake it hard.
• Once mixed, tape the cap firmly so it cannot come loose during mailing.

Proper cleaning after every session protects the accuracy of your next results. The most important rule: always finish with a distilled water rinse.

• Brix Refractometer: Open the lid and rinse the glass prism with distilled water. Pat dry gently with a soft cloth. Never submerge the whole instrument in water.
• EC20 Conductivity Tester: Rinse the probe thoroughly with distilled water and blot dry. If storing, add a few drops of distilled water inside the probe cap to keep the sensor hydrated.
• Pipettes: Disposable pipettes should be discarded. If reusing, rinse thoroughly with distilled water several times and air dry completely.
• Cups and test tubes: Rinse with distilled water several times and air dry upside down before storing.
• If any tool touched the baking soda solution: Rinse thoroughly to remove all baking soda residue, then rinse again with distilled water. Any baking soda left on a tool will affect your next reading.

Start by collecting your urine sample, then immediately prepare the well plate so it can begin developing while you run the other tests.

• Put on your medical gloves. Collect a fresh urine sample into a clean specimen cup.
• Get a clean test tube and pipette. Hold the pipette completely vertical. Place exactly 1 drop of urine into the test tube.
• Add exactly 6 drops of Universal Extraction Solution to the test tube.
• Using another clean pipette, mix by repeatedly squeezing the bulb in and out for approximately 1 full minute.
• Get your well plate. Place 1 drop of the mixture into each of 3 depressions on the top row, and 1 drop into each of 3 depressions on the bottom row — 6 drops total.

Start the timer as soon as you finish adding the drops, then move on to run the other tests. When the 4 minutes are up, a full-screen prompt will appear automatically with the next instructions no matter which step you are on.

• Set up the baking soda bowl: 1 teaspoon baking soda in a small bowl with ½ cup of water.
• Carefully open the Nitrate Test Solution bottle and extract a small amount with a clean pipette.
• For each of the 3 top-row depressions: place 1 drop on each of the 4 sides — not the center. Let each drop roll gently down the wall into the solution.
• Immediately place the used pipette into the baking soda bowl.
• Press Start on the timer below, then continue to your other tests.

Run this test while your nitrate timer is counting down.

• Open the lid completely so it lays flat on the opposite side.
• Using a clean pipette, place a thin layer of urine on the glass window and close the lid gently.
• Hold the viewing end toward a light source and look through the eyepiece.
• Read the number where the blue and white sections meet. Each small line equals 0.2.
• Rinse the glass with distilled water only and pat dry.

Run this test while your nitrate timer is still counting down.

• Tear off about 2 inches of the 5.5–8.0 pH paper.
• Dip it into your urine specimen cup and remove it immediately.
• Compare the color to the chart on the container right away. Do not wait.
• If the color matches exactly at 5.5, also dip the 3.0–5.5 strip to check lower.

Read the color immediately after removing the strip — do not wait.

• Tear off a 1 to 1½ inch piece of the 5.5–8.0 pH paper.
• Build up saliva, insert the strip, close your lips, remove immediately — or spit into a cup and dip.
• Immediately compare the color to the chart. Do this right away.
• If the color matches at exactly 5.5, also dip the 3.0–5.5 strip.

• Get a clean cup and place it against a wall or something sturdy.
• Using your pipette, draw urine up to the 2 ml mark and empty it into the cup.
• Measure exactly 70 ml of distilled water and pour it into the cup.
• Turn on the EC20, place the probe in the cup, give it a gentle stir, then hold it still. Do not hold it by hand.
• Wait approximately 1 minute for the reading to stabilize.
• Rinse the probe with distilled water only when finished.

• First look without any light. If you can already see cloudiness or floating particles, your reading is at least 4M+.
• Shine your phone flashlight through the side of the cup and look through from the other side.
• Match what you see to the guide below, then tap Enter my reading.

Proper cleaning after every session protects the accuracy of your next results. Always finish with a distilled water rinse.

• Brix Refractometer: Rinse the glass prism with distilled water and pat dry. Never submerge the whole instrument in water.
• EC20 Conductivity Tester: Rinse the probe with distilled water and blot dry. Add a few drops of distilled water inside the probe cap before storing.
• Well plate: Rinse each depression thoroughly with distilled water to remove all chemical residue. Air dry completely before storing.
• Pipettes, cups, and test tubes: Rinse with distilled water several times and air dry completely.
• If any tool touched the baking soda solution: Rinse all baking soda off first, then do a final distilled water rinse. Any residue will affect your next reading.